Journal: Cell Death Discovery
Article Title: Targeting matrix metallopeptidase 2 by hydroxyurea selectively kills acute myeloid mixed-lineage leukemia
doi: 10.1038/s41420-022-00989-4
Figure Lengend Snippet: a Immunoblots of MLL (MLL C180 ) in the indicated cell lines. b ChIP analyses at the promoter region of the MMP2 locus in the SKM1 and THP1 cells. * P < 0.05; ** P < 0.01; the ns indicate no significant difference; two-tailed t- test. Data represent the means of triplicate reactions ± SD. c Cell viability of SKM1 was measured after 72-hour exposure to HU with co-treatment of 50 µM MM-102. d Cell viability was measured after 72-h exposure to triapine with co-treatment of 20 µM ilomastat. The IC 50 of different cells was quantified. e Cell viability of indicated cells with co-treatment of 50 µM HU and 150 nM triapine after 72 h. f immunofluorescence visualized the localization of endogenous MMP2 in SKM1 cells. Scale bar, 5 μm. g Venn diagram shows the number of candidates who interacted with MMP2 in SKM1 cells without or with the treatment of 100 μM HU after 24 h. h , i GO enrichment ( h ), and GSEA analysis ( i ) of the common 39 interaction candidates of MMP2 in SKM1 cells with or without HU treatment. j THP1 cells were transfected with full-length MMP2, with a 3× FLAG-tag. Cell fractions were prepared for the Co-IP assays using the indicated antibodies. k Relative protein synthesis of SKM1 and THP1 cells with HU treatment after the indicated hours.
Article Snippet: SKM1 (JCRB cell bank, Osaka, Japan).
Techniques: Western Blot, Two Tailed Test, Immunofluorescence, Transfection, FLAG-tag, Co-Immunoprecipitation Assay
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